타액선 종양세포주에서 제2형 트랜스글루타민아제와 Nuclear Factor Kappa B의 연관성
Transgluraminase 2 Expression Was Associated with Nuclear Factor Kappa in Salivary Gland Tumor Cell Line
김인수, 박경주, 이종헌,
소속 상세정보
김인수 ( Kim In-Soo ) - 단국대학교 치과대학 구강병리학교실
박경주 ( Park Gyeong-Ju ) - 단국대학교 치과대학 구강병리학교실
이종헌 ( Lee Chong-Heon ) - 단국대학교 치과대학 구강병리학교실
KMID : 0829220100340020057
Abstract
The transcription factor nuclear factor-kB (NF-kB) plays an important role in regulating cell growth, apoptosis, and metastatic functions. Constitutive activation of NF-KB has been observed in various cancers; however, molecular mechanisms resulting in such activation remain elusive. Numerous evidences showed that over expression of TGase 2 might be linked with constitutive activation of NF-KB. To understand the pathways responsible for constitutive activation of NF-kB is important for rational design of NF-kB inhibitors for cancer therapy. Human salivary gland adenocarcinoma has been most aggressive solid tumors. Current therapy does not significantly improve survival rates. Thus, to investigate new therapeutic modalities against this adenocarcinoma is necessary. The purpose of this study was to study a constitutive activation of NF-kB with the expression of TG2 in SGT cell line origianted from human salivary gland adenocarcinoma by TGase 2 activity, RT-PCR and immunoslot blotting method. SGT cell line showed the highest TGase 2 activity and NF-kB activation than any other cell line. All the cell lines showed increased NF-kB mRNA activation after A231027 treatment than that of control. In immunoslot blotting, SGT cell line showed NF-kB activation correlated with TGase 2 expression after A231027 and BSA. It suggested that there might be a direct correlation between TGase 2 expression and NF-kB activation in SGT cell line.
키워드
SGT Cell Line; NF Kappa; TGase 2
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